Journal: bioRxiv

Article Title: Development of high-affinity, single-domain protein binders for neutralizing household allergens

doi: 10.1101/2025.08.03.668213

Figure Lengend Snippet: (a) Thermostability profile of AVA-1-1-C3 as a representative protein, as determined by differential scanning fluorimetry. (b-g) Cytotoxicity of primary human cells following 48-hour exposure to AVA-1-1-C3 or an equivalent concentration of bovine serum albumin (BSA), evaluated using the CellTiter-Glo® (CTG) luminescent cell viability assay. Cell types included human epithelial keratinocytes (HEK, b), human dermal fibroblasts (HDF, c), human follicle dermal papilla cells (HFDPC, d), human renal cortical epithelial cells (HRCEPC, e), human skeletal muscle cells (HSKMC, f), and human dermal microvascular endothelial cells (HDMEC, g). No significant differences in cell viability were observed. Data in (b-g) represents mean ± s.d. and are representative of two experimental replicates.

Article Snippet: For in vitro toxicity studies, primary cells including human dermal fibroblasts (HDF, Cell Applications, Cat# 106K-05a, lot 1632), human epidermal keratinocytes (HEK, Cell Applications, Cat# 102-05a, lot 2146), human dermal microvascular endothelial cells (HDMEC, PromoCell, Cat# C-12210, lot 483Z001.3), human skeletal muscle cells (HSKMC, Cell Applications, Cat# 150K-05a, lot 3507), and human renal cortical epithelial cells (HRCEpC, Promocell, Cat# C-12660, lot 501Z019.23).

Techniques: Concentration Assay, Cell Viability Assay

Journal: Experimental and Therapeutic Medicine

Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis

doi: 10.3892/etm.2019.8049

Figure Lengend Snippet: LncRNA MEG3 regulates renal epithelial cell and cardiomyocyte apoptosis following LPS treatment. LncRNA MEG3 overexpression and knockdown were confirmed. Following LPS treatment, LncRNA MEG3 overexpression resulted in significantly increased apoptosis, whereas lncRNA MEG3 knockdown by siRNA significantly inhibited apoptosis in (A) renal epithelial cells and (B) AC12 cardiomyocytes. *P<0.05. LncRNA, long non-coding RNA; MEG3, maternally expressed 3; LPS, lipopolysaccharide.

Article Snippet: Human primary renal mixed epithelial cells (ATCC ® PCS-400-012TM) were purchased from American Type Culture Collection.

Techniques: Over Expression, Knockdown

Journal: bioRxiv

Article Title: Development of high-affinity, single-domain protein binders for neutralizing household allergens

doi: 10.1101/2025.08.03.668213

Figure Lengend Snippet: (a) Thermostability profile of AVA-1-1-C3 as a representative protein, as determined by differential scanning fluorimetry. (b-g) Cytotoxicity of primary human cells following 48-hour exposure to AVA-1-1-C3 or an equivalent concentration of bovine serum albumin (BSA), evaluated using the CellTiter-Glo® (CTG) luminescent cell viability assay. Cell types included human epithelial keratinocytes (HEK, b), human dermal fibroblasts (HDF, c), human follicle dermal papilla cells (HFDPC, d), human renal cortical epithelial cells (HRCEPC, e), human skeletal muscle cells (HSKMC, f), and human dermal microvascular endothelial cells (HDMEC, g). No significant differences in cell viability were observed. Data in (b-g) represents mean ± s.d. and are representative of two experimental replicates.

Article Snippet: Cells were cultured in their corresponding complete media including HDF growth medium (Cell Applications, Cat# 116-500), human EpiVita serum-free growth medium (Cell Applications, Cat# 141-500a), microvascular endothelial cell growth medium (PromoCells, Cat# C-22120), HSkMC growth medium (Cell Applications, Cat# 151-500), and renal epithelial cell growth media (PromoCell, Cat# C-26130).

Techniques: Concentration Assay, Cell Viability Assay